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- The Zambales Ophiolite range, located in the Luzon province of the Philippines, hosts several continental serpentinizing fluid seeps in a tropical, densely vegetated environment. Fluid and sediment samples were collected for metagenomic analysis at Manleluag National Park to characterize the deep subsurface microbial community originating in these seeps. This study aims to compare the planktonic microbial deep subsurface community, collected via peristaltic pumping approximately 20 liters of fluid onto Sterivex filters, with sediment-bound microbial communities collected at the source of the seep. Additionally, samples isolated from adjacent springs will be analyzed to provide an indication of the genetic relatedness between two serpentinizing fluid seeps in Manleluag National Park. The results of this analysis will have relevance and be complimentary to studies by the broader deep life community, and will provide baseline data regarding the function and diversity of microbial communities in deeply-sourced tropical serpentinizing fluid seeps.
- November 2014 - November 2016