- Single-Cell Genomics - Subsurface Microbes
A large challenge in environmental microbiology is linking taxonomic marks to physiology and metabolic functions. Single-cell genomics provides unequivocal linkage information for multiple genes and enables the reconstruction of entire genomes from uncultured microbial species. This project will undertake genomic sequencing of individual cells from deep subsurface microbes in order to (1) reconstruct metabolic properties of predominant microbial types in studied communities, (2) serve as references when interpreting metagenomic and metatranscriptomic data, and (3) reveal evolutionary processes for deep subsurface microbial populations.The Deep Fracture (DF) portion of the Rock Hosted Communities Project sought to build upon previous studies on the microbial diversity of Precambrian Shield deep ecosystems and use cell sorting and single-cell genome sequencing approaches to evaluate the adaptations and the population genetics of these deep isolated communities. The work built primarily upon sampling opportunities in South Africa and Finland where populations of the deep biosphere organism Candidatus Desulforudis audaxviator were found to be prevalent.