Uncultured D esulfobacteraceae and Crenarchaeotal group C3 incorporate 13 C-acetate in coastal marine sediment Journal Article uri icon

DCO ID 11121/1802-4951-6115-1211-CC

is Contribution to the DCO

  • YES

year of publication

  • 2015

abstract

  • Stable isotope probing (SIP) of deoxyribonucleic acid (DNA) was used to identify microbes incorporating 13C-labeled acetate in sulfate-reducing sediment from Aarhus Bay, Denmark. Sediment was incubated in medium containing 10 mM sulfate and different 13C-acetate (10, 1, 0.1 mM) concentrations. The resultant changes in microbial community composition were monitored in total and SIP-fractionated DNA during long-term incubations. Chemical analyses demonstrated metabolic activity in all sediment slurries, with sulfate-reducing activity largely determined by initial acetate concentrations. Sequencing of 16S rRNA gene PCR amplicons showed that the incubations shifted the bacterial but not the archaeal community composition. After 3 months of incubation, only sediment slurries incubated with 10 mM 13C-acetate showed detectable 13C-DNA labeling. Based on 16S rRNA and dsrB gene PCR amplicon sequencing, the 13C-labeled DNA pool was dominated by a single type of sulfate reducer representing a novel genus in the family Desulfobacteraceae. In addition, members of the uncultivated Crenarchaeotal group C3 were enriched in the 13C-labeled DNA. Our results were reproducible across biological replicate experiments and provide new information about the identities of uncultured acetate-consuming bacteria and archaea in marine sediments.

volume

  • 7

issue

  • 4